Reagent Kit for Single Cell Gel Electrophresis Assay
产品先容:
DNA 在自由基(如·OH)的攻击下容易爆发损伤,即脱氧戊糖遭到破环,磷酸二脂键的断裂,碱基的破环或脱落,便可进一步爆发单链断裂或双链断裂。将细胞牢靠于低熔点琼脂糖中,取少量细胞涂在载玻片上,用碱高盐溶液破损细胞膜,再用碱溶液使 DNA 分子解旋。将载玻片置于电泳液中,在电场的作用下,DNA 分子向阳极移动。若是DNA 损伤严重,碎片多,则电泳速率快。未受损伤的DNA 大分子则由于细胞膜的阻隔,滞留在原处。用PI 染色或银染,可视察到DNA 受损的细胞形同彗星征象,作定性剖析。也可用相关的软件作定量剖析。
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