人白介素1β(IL1b)酶联免疫吸附检测试剂盒-金狮贵宾会3420055

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人白介素1β(IL1b)酶联免疫吸附检测试剂盒

ELK1270

规格：	价钱：
48T	￥1680.00
96T	￥2400.00

下载说明 ①

Overview 文献

Product name:	Human IL1b(Interleukin 1 Beta) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P01584
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

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标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.207	2.125
500.00	1.613	1.531
250.00	1.115	1.033
125.00	0.757	0.675
62.50	0.421	0.339
31.25	0.297	0.215
15.63	0.195	0.113
0.00	0.082	0.000

细密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

接纳率

Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	79-93%	86%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.