大鼠胰岛素(INS)酶联免疫吸附检测试剂盒-金狮贵宾会3420055

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大鼠胰岛素(INS)酶联免疫吸附检测试剂盒

ELK2370

规格：	价钱：
48T	￥1960.00
96T	￥2800.00

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Overview 文献

Product name:	Rat INS(Insulin) ELISA Kit
Reactivity:	Rat
Alternative Names:	Insulin
Assay Type:	Sandwich
Sensitivity:	4.93 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INS in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOMEDICINE & PHARMACOTHERAPY

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Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats

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Phytomedicine Plus

Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

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Journal of Pharmacy and Pharmacology

Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis

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Experimental Gerontology

Polyphenolic-rich Cissus quadrangularis extract ameliorates insulin resistance by activating AdipoR1 in peri-/post-menopausal rats

日期：2022-01-05 IF：4.032
Adipocyte

The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats

日期：2022-01-22 IF：4.534
Life Sciences

Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

日期：2020-04-24 IF：5.037
Phytomedicine Plus

Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

日期：2021-01-06 IF：-
Experimental Gerontology

Hesperidin inhibits NOX4 mediated oxidative stress and inflammation by upregulating SIRT1 in experimental diabetic neuropathy

日期：2022-12-19 IF：4.253
International Journal of Molecular Sciences

The Effects of Prenatal Dexamethasone Exposure on Brain Metabolic Homeostasis in Adulthood: Implications for Depression

日期：2023-01-06 IF：6.208
Biochemical Pharmacology

miR-486-5p-rich extracellular vesicles derived from patients with olanzapine-induced insulin resistance negatively affect glucose-regulating function

日期：2024-05-22 IF：5.8
Nigerian Journal of Clinical Practice

Effect of the Combination of Exercise and Metformin on Osteocalcin, Insülin, Interleukin-6, Glucose Levels, and Body Weights in Rats

日期：2024-06-15 IF：0.7
Journal of Nutritional Biochemistry

Luteolin alleviates muscle atrophy, mitochondrial dysfunction and abnormal FNDC5 expression in high fat diet-induced obese rats and palmitic acid-treated C2C12 myotubes

日期：2024-10-11 IF：4.8
Journal of Nutritional Biochemistry

Luteolin alleviates muscle atrophy, mitochondrial dysfunction and abnormal FNDC5 expression in high fat diet-induced obese rats and palmitic acid-treated C2C12 myotubes

日期：2024-12-09 IF：4.8
Journal of Nutritional Biochemistry

Luteolin alleviates muscle atrophy, mitochondrial dysfunction and abnormal FNDC5 expression in high fat diet-induced obese rats and palmitic acid-treated C2C12 myotubes

日期：2024-12-09 IF：4.8
Journal of Nutritional Biochemistry

Luteolin alleviates muscle atrophy, mitochondrial dysfunction and abnormal FNDC5 expression in high fat diet-induced obese rats and palmitic acid-treated C2C12 myotubes

日期：2024-12-09 IF：4.8

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.035	1.940
500.00	1.621	1.526
250.00	1.152	1.057
125.00	0.887	0.792
62.50	0.526	0.431
31.25	0.367	0.272
15.63	0.183	0.088
0.00	0.095	0.000

细密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

接纳率

Matrices listed below were spiked with certain level of recombinant INS and the recovery rates were calculated by comparing the measured value to the expected amount of INS in samples.

Matrix	Recovery range	Average
serum(n=5)	92-106%	99%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	78-92%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.