大鼠白介素17(IL17)酶联免疫吸附检测试剂盒-金狮贵宾会3420055

产品中心

大鼠白介素17(IL17)酶联免疫吸附检测试剂盒

ELK2558

规格：	价钱：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview 文献

Product name:	Rat IL17(Interleukin 17) ELISA Kit
Reactivity:	Rat
Alternative Names:	IL-17;IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Assay Type:	Sandwich
Sensitivity:	6.3 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL17 in the samples is then determined by comparing the OD of the samples to the standard curve.

Chemico-Biological Interactions

Wnt/β-catenin antagonist pyrvinium rescues high dose isoproterenol induced cardiotoxicity in rats: Biochemical and immunohistological evidences

日期：2022-03-18 IF：5.194
Behavioural Brain Research

Comparative evaluation of different chemical agents induced Autism Spectrum Disorder in experimental Wistar rats

日期：2023-10-18 IF：2.7
European Journal of Pharmacology

Fucoxanthin mitigates valproic acid-induced autistic behavior through modulation of the AKT/GSK-3β signaling pathway

日期：2024-03-15 IF：5
European Journal of Pharmacology

Modulation of SIRT6 related signaling pathways of p-AKT/mTOR and NRF2/HO-1 by memantine contributes to curbing the progression of tamoxifen/HFD-induced MASH in rats

日期：2024-12-09 IF：4.2
European Journal of Pharmacology

Modulation of SIRT6 related signaling pathways of p-AKT/mTOR and NRF2/HO-1 by memantine contributes to curbing the progression of tamoxifen/HFD-induced MASH in rats

日期：2024-12-09 IF：4.2
European Journal of Pharmacology

Modulation of SIRT6 related signaling pathways of p-AKT/mTOR and NRF2/HO-1 by memantine contributes to curbing the progression of tamoxifen/HFD-induced MASH in rats

日期：2024-12-09 IF：4.2

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.121	2.056
500.00	1.694	1.629
250.00	1.276	1.211
125.00	0.892	0.827
62.50	0.595	0.530
31.25	0.387	0.322
15.63	0.100	0.035
0.00	0.065	0.000

细密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

接纳率

Matrices listed below were spiked with certain level of recombinant IL17 and the recovery rates were calculated by comparing the measured value to the expected amount of IL17 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	85-99%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.