½ðʨ¹ó±ö»á3420055

½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ ½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ

ÊÖÒÕרÀ¸

Äڲο¹ÌåÔõôѡ
½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ¹©¸å£ºÊÖÒÕ²¿½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕÐû²¼Ê±¼ä£º2022-05-11½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕä¯ÀÀÁ¿£º1316´Î

Äڲο¹Ìå¿ÉÓÃÓÚÆÀ¼ÛÂÑ°×ÖÊÃâÒßÓ¡¼£·¨(Western Blot)µÄЧÂÊ£¬½ÏÁ¿Äý½ºÖÐÿ¿×µÄÂÑ°×ÉÏÑùÁ¿¡£´ËÀà±ÈÕÕ¿É×ÊÖúÈ·¶¨ÑùÆ·¼ä±í´ïˮƽµÄ²î±ð£¬ÒÔÅжÏÊÇÓÉϸ°ûÁѽâÎïµÄÏÖʵÂÑ°×ˮƽµ¼Ö£¬ÕÕ¾ÉÓÉÉÏÑùÁ¿µÄ²î±ðµ¼Ö¡£

Äڲο¹ÌåÖ÷Òª¹¦Ð§

£¨1£©¼ì²âÕû¸öWBÁ÷³ÌÊÇ·ñÕý³£

£¨2£©¼ì²â»ùÒò±í´ï²úÆ·ÊÇ·ñ׼ȷ

£¨3£©½ÏÁ¿²úƷ΢Á¿µÄÏà¶Ôת±ä

£¨4£©ÆÀ¼Û¸÷¸öÉÏÑù¿×ÄÚÂÑ°×µÄ×ÜÁ¿ÊÇ·ñ»ù±¾Ò»ÖÂ

ͼƬ

ͼһʾÀý

Äڲο¹ÌåÔÚÄ¿µÄÂÑ°×µÄÃâÒßÓ«¹âÑо¿ÖУ¬Ò²¿ÉÒÔ×÷Ϊ¹²È¾µÄÑôÐÔ±ÈÕÕ¡£Äڲο¹Ìå·ÖΪδ±ê¼ÇÐͺͱê¼ÇÐÍ£¬±ê¼ÇÎï°üÀ¨ÉúÎïËØ¡¢HRP¡¢FITC¡¢PE¡¢CyȾÁϺÍAlexa FluorȾÁÏ¡£

Äڲο¹ÌåÖÖÀàÐí¶à£¬ºÃ±Èβ-actin¡¢β-tubunlin¡¢GAPDH¡¢Vinculin¡¢LaminB¡¢PCNAµÈ£¬¶Ô²î±ðÑù±¾Ñ¡Ôñ·×ÆçÑùµÄÄڲο¹ÌåÒ²ÊÇWBʵÑéÀֳɵÄÒªº¦¡£±¾ÎĽ«¼òÆÓÏÈÈÝÑ¡ÔñÄڲο¹ÌåÓ¦×ñÕÕµÄÎåÏîÔ­Ôò£º

 

Ò»¡¢Ñù±¾ÖÖÊôȪԴ£º

Ê×ÏÈҪ˼Á¿ÊµÑéÑùԭȪԴÓÚʲôÎïÖÖ¡£

 

1. ²¸È鶯ÎïµÄ×éÖ¯»òÕßϸ°ûÑù±¾£¬Í¨³£Ñ¡Ôñβ-actin¡¢β-tubulin¡¢GAPDH¡¢Lamin B¡¢Histone H3¡¢Na,KatpaseµÈ¡£

 

2. Ö²ÎïȪԴʵÑéÑù±¾£¬Ôò¿ÉÒÔÑ¡Ôñplantactin¡¢RubiscoµÈ¡£

 

3. ÆäËûȪԴÑù±¾Ñо¿½ÏÉÙ£¬ÒÔÊǾÍÓ¦¸Ã²ÎÕÕÆÚ¿¯ÎÄÏ×Êý¾Ý£¬Ñ¡ÔñºÏÊʵÄÂÑ°××÷ΪÄڲΡ£

 

¶þ¡¢·Ö×ÓÁ¿´óС£º

Ñ¡ÔñÄڲο¹Ìåʱ£¬Ó¦¸Ã˼Á¿Ä¿µÄÂÑ°×·Ö×ÓÁ¿µÄ¾Þϸ£¬Ò»Ñùƽ³£°ü¹ÜÄ¿µÄÂÑ°×ÓëÄÚ²ÎÂÑ°×·Ö×ÓÁ¿Ïà²î5KDÒÔÉÏ¡£ÀýÈ磬ĿµÄÂÑ°×·Ö×ÓÁ¿Îª40KD£¬´Ëʱ²»ÏàÒËÑ¡Ôñβ-actin¡¢ÓëGAPDH×÷ΪÄڲΣ¬¿ÉÒÔ˼Á¿Ñ¡Ôñα-Tubulin»òÕßβ-tubulin×÷ΪÄڲΡ£

 

Èý¡¢±í´ïˮƽ£º

Ñ¡ÔñµÄÄÚ²ÎÂÑ°×ÐèÒªÔÚ´ý²âÑù±¾ÖÐÊǸ߱í´ïµÄ¡£³£ÓõÄÄÚ²ÎÂÑ°×ÊÇÓɹܼһùÒò¸ß±í´ïµÄ£¬ÊÇϸ°û·¢ÓýºÍά³Öϸ°û»îÁ¦Ëù±ØÐèµÄ¡£ÀýÈ磬ϸ°ûºËµÄÄÚ²ÎÂÑ°× PCNA£¬ÔÚ DNA S ÆÚ±í´ï£¬Òò´Ë¹ØÓÚ·ÇÔöֳϸ°û²»ÍƼöʹÓá£

 

ËÄ¡¢Ä¿µÄÂÑ°×±í´ï¶¨Î»£º

ͼƬ

 

 

ͼƬ

 

Îå¡¢ELK Biotechnology¿ÉÑ¡Ïà¹ØÄڲΣº

ÊÊÓùæÄ£ Ãû³Æ »õºÅ ·Ö×ÓÁ¿
°ûÖÊ β-Tubulin EM1114 50kDa
β-Actin EM1031 43kDa
α-Tubulin EM1115 52kDa
GAPDH EM1028 37kDa
Vinculin ES3700 123kDa
Cyclophilin B EM1219 21kDa
ºËĤÂÑ°× Lamin B EM1035 68kDa
ºËÂÑ°× PCNA EM1030 29kDa
TATA binding protein TBP/TBP EM1216 38kDa
YY1 ES11233 45kDa
HDAC1 ES4225 55kDa
Histone H3 EM1037 15kDa
ĤÂÑ°× ATPase Na+/ K+ beta 2 (ATPB2) / 112kDa
ÖÊĤ ATP1A1 (Sodium Potassium ATPase) ES2892 100kDa
ÏßÁ£Ìå COX4 EM1032 15kDa
VDAC1 ES4120 31kDa

ȫѪ

Ѫ½¬

ѪÇå

Transferrin EM1231 77kDa
Albumin EM1190 66kDa
Ö²ÎïÄڲΠPlant actin EM1147 43kDa
¹Ø±Õ

ÔÚÏß×Éѯ

Online consultation

  • ½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ ÔÚÏß×Éѯ
  • ½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ ÊÖÒÕÖ§³Ö
½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ

¹Øע΢ÐŹ«ÖÚºÅ

½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ

΢ÐÅɨһɨÁ¬Ã¦×Éѯ

΢ÐŵçÄÔ¶Ë×Éѯ
½ðʨ¹ó±ö»á-±öÖÁÈç¹é-×ð¹ó-ÏÔºÕ

΢ÐÅɨһɨÁ¬Ã¦×Éѯ

΢ÐŵçÄÔ¶Ë×Éѯ
ÍøÕ¾µØͼ
ÓÑÇéÁ´½Ó£ºk8¿­·¢¹ÙÍøÈë¿Ú  ÖDz©1919ÌåÓý  jdbµç×ÓÓÎϷƽ̨  SGÑÇÖÞʤÓΠ ¿­Ê±¹Ù·½  cq9µç×Ó  orangeƽ̨  pgµç×Ó  ok138Ì«Ñô¼¯ÍÅ  ¹·ÍòÌåÓý  ±¦ÂíÓéÀÖÍøÕ¾bmw0002  orangeƽ̨  pgÉͽðÅ®ÍõÄ£ÄâÆ÷ÔÚÏßÊÔÍæ  ÌìÓεǼÖÐÐÄ  ±ØÒ»Ô˶¯